Mitochondrial-Targeted Two-Photon Fluorescent Probes for Zinc Ions, H_𝟐O_𝟐, and Thiols in Living Tissues

Figure 4

Ratiometric TPM images of a rat hippocampal slice stained with (a) 20 μM SHP-Mito and (c) 1-SHP and (b) pretreated with 1 mM H₂O₂ for 30 min before labeling with 20 μM SHP-Mito. Ten ratiometric TPM images were accumulated along the z-direction at the depths of approximately 90–180 μm with magnification at 10x. (d–f) Enlarged images of (a–c) at 120 μm depth with 40x magnification. (g) Approximate positions (dotted circles) used for measurements of emission ratios in the CA3 and CA1. (h) Average 𝐹_yellow/𝐹_blue in (a–c). The TPEF was collected at two channels (blue = 400–470 nm and yellow = 530–600 nm) upon excitation at 750 nm with fs pulse. Scale bars: 300 μm (a) and 75 μm (d) [11].

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