Mitochondrial-Targeted Two-Photon Fluorescent Probes for Zinc Ions, , and Thiols in Living Tissues
Figure 6
Images of a rat hippocampal slice stained with 20 μM SSH-Mito for 2 h. (a and d) Bright-field images of the CA1-CA3 regions. (b and e) Ratiometric TPM images of a fresh rat hippocampal slice (b) nontreated and (e) pretreated with NEM (100 μM) for 30 min before labeling with SSH-Mito. Ten ratiometric TPM images were accumulated along the z-direction at the depths of approximately 90–190 μm with magnification at 10x. (c and f) Enlarged images of red box in (b) and (e) at 120 μm depth with 40x magnification. The TPEF was collected at two channels (blue = 425–475 nm and yellow = 525–575 nm) upon excitation at 740 nm with fs pulse. Scale bars: 300 μm (a and d) and 75 μm (c and f) [12].
