Curcumin Protects Human Keratinocytes against Inorganic Arsenite-Induced Acute Cytotoxicity through an NRF2-Dependent Mechanism
Figure 1
Curcumin activates ARE in HaCaT cells. (a) Cytotoxicity of curcumin and tBHQ. HaCaT cells were exposed to different concentrations of curcumin or tBHQ for 24 hr, and then cell viability was measured by MTT assay. Data are expressed as mean ± SD. . * versus vehicle (medium). (b) Curcumin activates ARE in a concentration- and time-dependent manner. Cultured HaCaT cells stably transduced with the ARE-luciferase reporter were exposed to 40 μM tBHQ for indicated period of time as a positive control (left panel); then ARE activation was detected in the condition of different concentrations of curcumin for 6 hr (middle panel) or 2.5 and 5 μM of curcumin for indicated period of time (right panel). Values shown are mean ± SD. . * versus vehicle (medium).
