Enhanced 4-Hydroxynonenal Resistance in KEAP1 Silenced Human Colon Cancer Cells
Figure 7
Effect of AKR1C on 4HNE protein adducts formation and cytotoxicity by 4HNE. (a) Cell viabilities were determined in the sc and shKEAP1 HT29 cells following the incubation with 4HNE (10–160 μM) for 24 h. (b) Cell viabilities were monitored following the incubation with 40 μM 4HNE for 48 h. The values are relative levels with respect to each vehicle group and are the means ± SD of 8 wells. compared with the sc control cell line. (c) The sc and shKEAP1 HT29 cells were incubated with 4HNE (160 μM) for 0.5 and 1 h and the levels of 4HNE adducts were monitored using western blot analysis. The bar graph represents quantified intensities of 4HNE adducts/β-tubulin. Average total intensities of 4HNE adducts were measured and normalized with each β-tubulin intensity. compared with the sc control cell line. (d) The sc and shKEAP1 HT29 cells were coincubated with flufenamic acid (Flu, 20 μM) and 4HNE (40 μM) for 3 h. The levels of 4HNE protein adducts were measured in cell lysates from the sc and shKEAP1 HT29 cells. The bar graph represents quantified intensities of 4HNE adducts/β-tubulin. compared with the sc control cell line. (e) The cell viabilities were assessed following the coincubation with flufenamic acid (F) and 4HNE. The values are relative levels with respect to each vehicle group and are the means ± SD of 8 wells. compared with the sc control cell line. compared with 4HNE treated scHT29 cells.
