Early Growth Response Protein 1 Promotes Restenosis by Upregulating Intercellular Adhesion Molecule-1 in Vein Graft

Figure 4

Egr-1 knockout (KO) decreased ICAM-1 expression. (a) Venous ECs from WT and Egr-1 KO mice were isolated and stimulated with mechanical stretch from 0 to 3 h (𝑛=5). ICAM-1 mRNA expression was determined by real-time RT-PCR. (b) Egr-1 KO decreased ICAM-1 protein levels after mechanical stretch stimulation for 24 h (𝑛=5). Data are expressed as mean ± SEM. *𝑃<0.05 versus WT group; ^#𝑃<0.05 versus WT/ST group. (c) Egr-1 KO decreased ICAM-1 mRNA expression in mouse vein graft model (𝑛=5). Data are expressed as mean ± SEM. *𝑃<0.05 versus WT group. WT: wild-type mice; WT/ST: venous ECs from WT mice stimulated with mechanical stretch; Egr-1 KO: Egr-1 knockout mice; Egr-1 KO/ST: venous ECs from Egr-1 knockout mice stimulated with mechanical stretch.

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