Research Article

Early Growth Response Protein 1 Promotes Restenosis by Upregulating Intercellular Adhesion Molecule-1 in Vein Graft

Figure 5

Immunohistochemistry of Egr-1 and ICAM-1 was performed in vein grafts from Egr-1 KO mice and WT mice at 4 weeks after surgery. (a) Vein grafts from Egr-1 KO mice and WT mice were stained with Egr-1 and ICAM-1 monoclonal antibody (×200). Brown colour indicates positive staining for all primary antibodies. (b) Quantitative analysis of the percentages of Egr-1 or ICAM-1 positive cells in vein grafts using NIS Elements BR software (NIKON, Japan). Data are expressed as mean ± SEM. # WT-EGR1 versus KO-EGR1 group; * WT-ICAM1 versus KO-ICAM1 group. WT-EGR1: vein grafts from WT mice were stained with Egr-1 monoclonal antibody; KO-EGR1: vein grafts from Egr-1 KO mice were stained with Egr-1 monoclonal antibody; WT-ICAM1: Vein grafts from WT mice were stained with ICAM-1 monoclonal antibody; KO-ICAM1: Vein grafts from Egr-1 KO mice were stained with ICAM-1 monoclonal antibody.
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