Sublethal Oxidative Stress Induces the Premature Senescence of Human Mesenchymal Stem Cells Derived from Endometrium
Figure 3
The sublethal dose of H2O2 induces senescent phenotype in hMESCs. Cells were treated as indicated in the legend of Figure 2. (a) SA--Gal staining. (b) Quantitative assay of SA--Gal-positive cells. (c) H2O2-induced cell size increase. Typical presentation of forward scatter (FS), reflecting the average cell size (left). Cell size was determined daily: H2O2-treated cells were either cultured for 5 days under standard conditions (middle) or were reseeded in 2 days and additionally cultivated for 3 days (right). Data were obtained by light-scattering cytometry with using Win MDI program version 2.8. Data represent mean ± SEM of at least three independent experiments. Significant difference was based on the Student’s -test (, ). Control (Ctr): untreated cells.
