Sublethal Oxidative Stress Induces the Premature Senescence of Human Mesenchymal Stem Cells Derived from Endometrium

Figure 6

The senescent phenotype in hMESCs treated with 900 μM H₂O₂. (a) H₂O₂-induced inhibition of cell proliferation (M ± SEM, 𝑛=3). (b) SA-𝛽-Gal staining. (c) Quantitative assay of SA-𝛽-Gal-positive cells. (d) Cell hypertrophy detected by light-scattering cytometry. Significant difference was based on the Student’s 𝑡-test (*𝑃<0.05, **𝑃<0.01, and ***𝑃<0.001). Control (Ctr): untreated cells.

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