Mitochondrial DNA Instability in Cells Lacking Aconitase Correlates with Iron Citrate Toxicity
Figure 1
The RTG pathway is activated in aco1Δ mutant cells grown in dextrose medium. Wild-type rho+ (WT, BY4741), its rho0 derivative, and aco1Δ (ZLY2603) mutant strains were transformed with a centromeric plasmid encoding a CIT2-lacZ reporter gene (pCIT2-lacZ) and transformants were grown in YNBcasR (Raffinose) and YNBcasD (Dextrose) medium to mid-logarithmic phase. Cells were collected and -galactosidase assays were conducted as described in Section 2.
