Mitochondrial DNA Instability in Cells Lacking Aconitase Correlates with Iron Citrate Toxicity

Figure 4

Mutations in MRS3 and MRS4, encoding mitochondrial iron transporters, prevent mtDNA loss due to aco1Δ. Cells grown in YPD medium to mid-logarithmic phase were stained with DAPI and examined using fluorescence microscopy. WT (rho⁺), BY4741; aco1Δ, ZLY2630; mrs3Δ mrs4Δ aco1Δ, ZLY3505.