Research Article

Mitochondrial DNA Instability in Cells Lacking Aconitase Correlates with Iron Citrate Toxicity

Table 2

Plasmids used in this study.

PlasmidDescriptionSource

pRS416A yeast centromeric plasmid carrying URA3 selection marker[30]
pUC-rtg1::LEU2An rtg1::LEU2 disruption cassette in pUC19[31]
pUC-rtg2::LEU2An rtg2::LEU2 disruption cassette in pUC19[31]
pUC-rtg3::URA3An rtg3::URA3 disruption cassette in pUC19[32]
pBS-aco1::HIS3An aco1::HIS3disruption cassette in pBluescriptThis study
pCIT2-lacZA CIT2-lacZ reporter gene on the plasmid pWCJ (CEN URA3)This study
pRS303-SOD1The SOD1 gene was cloned into the integrative plasmid pRS303This study