Hyperglycemia-Induced Inhibition of DJ-1 Expression Compromised the Effectiveness of Ischemic Postconditioning Cardioprotection in Rats

Figure 4

Immunoblot and densitometry analysis of the protein expression of DJ-1, PTEN, phosphorylated, and total Akt in normal and diabetic myocardial tissue. GAPDH was set as a loading control. Data are the mean ± SD (𝑛=6 each). (a) Western blot of DJ-1, PTEN, p-Akt, total Akt, and GAPDH. (b) Statistical analysis of DJ-1 among different groups. (c) Statistical analysis of PTEN among different groups. (d) Statistical analysis of p-Akt among different groups. (e) Statistical analysis of total Akt among different groups. ^▴𝑃<0.05 & ^▴▴𝑃<0.01 versus N + S group; ^∗𝑃<0.05 & ^∗∗𝑃<0.01 & ^∗∗∗𝑃<0.001 versus respective sham group; ^#𝑃<0.05 versus respective IR group; ^###𝑃<0.001 versus respective IR group.

(a)

(b)

(c)

(d)

(e)