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Oxidative Medicine and Cellular Longevity
Volume 2013 (2013), Article ID 574029, 12 pages
http://dx.doi.org/10.1155/2013/574029
Research Article

Resveratrol Prevents Dendritic Cell Maturation in Response to Advanced Glycation End Products

1Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, 299 Viale Regina Elena, 00161 Rome, Italy
2Department of Hematology, Oncology and Molecular Medicine, Istituto Superiore di Sanità, 00161 Rome, Italy
3Department of Pharmacology, Faculty of Pharmacy, Hacettepe University, 06100 Ankara, Turkey
4Department of Physiology and Pharmacology “Vittorio Erspamer”, La Sapienza University of Rome, 00185 Rome, Italy

Received 1 March 2013; Revised 6 June 2013; Accepted 7 June 2013

Academic Editor: Mahesh Thirunavukkarasu

Copyright © 2013 Brigitta Buttari et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Advanced glycation end products (AGEs), generated through nonenzymatic glycosylation of proteins, lipids, and nucleic acids, accumulate in the body by age thus being considered as biomarkers of senescence. Senescence is characterized by a breakdown of immunological self-tolerance, resulting in increased reactivity to self-antigens. Previous findings suggest that AGE and its receptor RAGE may be involved in the pathogenesis of autoimmune reactions through dendritic cell (DC) activation. The aim of this study was to investigate whether resveratrol, a polyphenolic antioxidant compound with tolerogenic effects on DCs, was able to counteract the mechanisms triggered by AGE/RAGE interaction on DCs. By immunochemical and cytofluorimetric assays, we demonstrated that in vitro pretreatment of human monocyte-derived DCs with resveratrol prevents DC activation in response to glucose-treated albumin (AGE-albumin). We found that resveratrol exerts an inhibitory effect on DC surface maturation marker and RAGE up-regulation in response to AGE-albumin. It also inhibited proinflammatory cytokine expression, allostimulatory ability upregulation, mitogen-activated protein (MAP) kinases, and NF- B activation in AGE-albumin-stimulated DCs. We suggest that resveratrol, by dismantling AGE/RAGE signaling on DCs may prevent or reduce increased reactivity to self-molecules in aging.