Resveratrol Prevents Dendritic Cell Maturation in Response to Advanced Glycation End Products

Figure 2

Flow cytometric analysis of phenotypic dendritic cell (DC) maturation. Five-day human DCs pretreated or not with resveratrol (Resv, 50 μM) were cultured for 18 hours with or without AGE-albumin (AGE, 30 μg/mL). DCs treated with LPS (0.2 μg/mL), albumin (Alb; 30 μg/mL), and resveratrol (Resv; 50 μM) were used as controls. Expression of surface molecules was analyzed by flow cytometry as described in Section 2. Phenotypic maturation of DCs was detected by the appearance of CD83 (a) and by the expression of surface molecules (b). After 18 hours of incubation LPS and AGE-albumin induced almost similar DC maturation. Pretreatment of iDCs with resveratrol prevented the phenotypic maturation of DCs induced by AGE-albumin (CD83 and HLA-DR: ^*†‡𝑃<0.001; CD40 and CD80: ^*†𝑃<0.001, ^‡𝑃<0.01; CD86: *𝑃<0.001, ^†‡𝑃<0.01). Results are expressed as positive cell percentages (a) and mean fluorescence intensity (MFI, B) (means ± SD, 𝑛=4). Samples were analyzed on a FACSCanto cytofluorimeter using CellDIVA (BD-Biosciences). 𝑃 values by one-way ANOVA with a Bonferroni post hoc test.

(a)

(b)