Resveratrol Prevents Dendritic Cell Maturation in Response to Advanced Glycation End Products

Figure 4

Cytokine production in dendritic cell (DC) culture supernatants. Five-day human DCs pretreated or not with resveratrol (Resv, 50 μM) were cultured for 18 hours with or without AGE-albumin (AGE, 30 μg/mL). DCs treated with LPS (0.2 μg/mL), albumin (Alb; 30 μg/mL), and resveratrol (Resv; 50 μM) were used as controls. Supernatants were collected after 18 hours to measure IL-12p70, TNF-α, IL-10, and IL-1β by specific ELISA experiments. LPS and AGE-albumin (AGE) triggered a statistically significant upregulation of all cytokine secretions. Pretreatment of iDC with resveratrol prevented the upregulation of all proinflammatory cytokines in response to AGE-albumin. Results are expressed as means ± SD of four independent experiments (IL-12p70: *𝑃<0.01, ^†‡𝑃<0.001; TNF-α and IL-1β: ^∗†‡𝑃<0.001, ^§𝑃<0.05; IL-10: ^∗†𝑃<0.001).