Mild Systemic Oxidative Stress in the Subclinical Stage of Alzheimer’s Disease

Figure 1

Representative flow cytometric analysis of whole blood samples stained with anti-CD11b-APC (right column) and anti-HLA-DR-PE (left column) monoclonal antibodies. From all events acquired in gate R1 (upper left graph), CD14-positive monocytes (gate R2, separated from CD66b-positive neutrophils in gate R3; upper right graph) were used for histogram calculation of the geometric mean fluorescence intensity (GMFI). A similar analysis was performed for the detection of fluorescent DCF in the ROS assay (histogram not shown). INT (sample from cognitively intact patient) and AD (sample from patient with probable Alzheimer’s disease).