A Regulatory Role of NAD Redox Status on Flavin Cofactor Homeostasis in S. cerevisiae Mitochondria
Figure 3
Fluorimetric evidence of -NAD+ cleavage catalyzed by solubilized SCM. (a) SCM (0.3 mg protein), solubilized with TX100, were added to -NAD+ (50 μM) in the standard medium and the reaction followed at 25°C, essentially as described in Section 2. -NAD+ emission spectra (at excitation wavelength 310) were monitored at different incubation times. (b) -NAD+ fluorescence decrease was continuously monitored at 310/410 nm under the same experimental condition described in (a) using intact (−TX100) or solubilized SCM (+TX100).
