A Regulatory Role of NAD Redox Status on Flavin Cofactor Homeostasis in S. cerevisiae Mitochondria
Figure 7
Endogenous FAD hydrolysis by isolated SCM induced by TX100; inhibition by NAD+. (a) SCM (0.1 mg protein) were incubated under the experimental conditions described in Section 2. Fluorescence emission spectra (excitation wavelength at 450 nm) of the suspension were recorded after 150 min in the absence (−TX100, dotted line) or in the presence of TX100 (0.1%). Where indicated, NAD+ (1 mM) was also added. In the inset, the fluorescence intensity, measured at 520 nm, as obtained after TX100 addition, either in the absence or in the presence of NAD+, was plotted against the incubation time. (b) FAD, FMN, and Rf in TX100 treated mitochondrial suspension extracts were revealed by means of HPLC measurements, performed as described in Section 2, following 0 (panel (A)) or 150 min incubation time in the absence (panel (B)) or presence (panel (C)) of NAD+ (1 mM).
