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Figure 3: Immunofluorescence localization of p70 in vascular smooth muscle cells. vSMCs were treated with vehicle or 0.3 or 3 μM BaP for 3 hr and then processed for immunofluorescence detection as described in Section 2. Panel (a)—morphometric analysis of total immunofluorescence signal before and after filtration for cellular signal. Panel (b)—morphometric analysis of total immunofluorescence signal before and after filtration for nuclear signal. Panel (c)—the interaction with N-acetylcysteine was examined in experiments where cultures were pretreated with 0.5 mM N-acetylcysteine and then challenged with 0.3 or 3 μM BaP or DMSO. Similar results were seen in 3 independent experiments.