628615.fig.007a
(a)
628615.fig.007b
(b)
Figure 7: Regulation of gene inducibility in vascular smooth muscle by albumin. vSMCs were grown in the presence of serum (DMEM plus serum), or in serum-free medium (ExCell 293). Panel (a) shows patterns of cyp1a1 inducibility by BaP in the presence or absence of extracellular albumin, ExCell 293 was supplemented with BSA (100 μg/mL) for and then challenged with BaP (3 μM) or vehicle (DMSO) for 24 hr. Total RNA was analyzed for cyp1a1 gene expression by RT-PCR and gel-electrophoresis. DNA ladder in basepairs (bp) was loaded at the extreme right. Similar profiles were seen in 4 independent experiments. Panel (b) shows patterns of redox regulated expression for NQO1, GSTA1 and Ha-ras in the presence or absence of extracellular albumin and BaP. Samples were processed as described above. Similar profiles were seen in 3 independent experiments.