Maintenance of Mitochondrial Morphology by Autophagy and Its Role in High Glucose Effects on Chronological Lifespan of Saccharomyces cerevisiae
Figure 2
Mitochondrial morphologies of S. cerevisiae grown in different concentrations of glucose. (a) BY4743 wild-type cells transformed with an ACO1-GFP fusion construct were grown for 72 hours in three different concentrations of glucose (0.5%, 2%, and 4%), and the morphology of mitochondria was observed at the indicated times using a fluorescent microscope. The micrographs shown are representative of the population. Scale bar: 5 μm. (b) The rates of oxygen consumption of these cells were also measured at the initial 6 hours after inoculation and at 12 hours intervals throughout the 72 hours time course. Data are the mean of three separate cultures in parallel. Error bars indicate the standard deviation.
