Maintenance of Mitochondrial Morphology by Autophagy and Its Role in High Glucose Effects on Chronological Lifespan of Saccharomyces cerevisiae
Figure 4
Conditioned medium from S. cerevisiae grown in 4% glucose triggered mitochondrial fragmentation, which was delayed by autophagy. (a) The wild-type cells pregrown to exponential phase for 6 hours in 0.5%, 2%, and 4% glucose to exponential phase were transferred to the 4% conditioned medium (4% 48 h) or 0.5% conditioned medium (0.5% 48 h), and mitochondrial morphology was observed. (b) The wild-type cells grown to stationary phase for 48 hours in 0.5%, 2%, and 4% glucose, then transferred to the 4% conditioned medium (4% 48 h) or 0.5% conditioned medium (0.5% 48 h), and mitochondrial morphology was observed. (c) Mutants affected in autophagy (Δatg1 and Δuth1) were grown to stationary phase as under (b), and mitochondrial morphology was observed. Scale bar: 5 μm. The micrographs shown are representative of the populations. Note: the micrograph for the Δuth1 mutant transferred into 4% 48 h represents the morphology of half the population of the cells, and the inset represents that of the other half of the population.
