Maintenance of Mitochondrial Morphology by Autophagy and Its Role in High Glucose Effects on Chronological Lifespan of Saccharomyces cerevisiae
Figure 5
Reversibility of conditioned medium-induced mitochondrial fragmentation. (a) The mitochondria of the wild-type cells grown in 2% glucose did not fragment when they were transferred into diluted (4% 48 h dilu) and evaporated (4% 48 h evap) conditioned media (4% glucose, 48 hours). Mitochondria were observed at 2 hours, 20 hours, and 62 hours after media exchange. Only the micrographs taken at 20 hours after medium exchange are shown. (b) Mitochondrial fragmentation of the wild-type cells grown in 2% glucose was triggered by the 4% conditioned medium (+4% 48 h). Cells were grown in the 4% conditioned medium for 24 hours before transferring into the 0.5% conditioned medium (+0.5% 48 h). The morphology of mitochondria was observed 2 hours after transfer. Scale bar: 5 μm. The micrographs shown are representative of the populations.
