Enhanced Prostacyclin Synthesis by Adenoviral Gene Transfer Reduced Glial Activation and Ameliorated Dopaminergic Dysfunction in Hemiparkinsonian Rats
Figure 2
NO synthesis and expressions of iNOS, COX-2, and PGIS in AdGFP- and AdPGIS-transduced mixed glia cultures before and after LPS challenge. (a) Western blot analysis of protein levels of PGIS, COX-2, and iNOS in cultures receiving AdGFP or AdPGIS infection. After 3 days of Ad infection, cultures were treated with LPS (600 ng/mL) for 2 days and harvested. Equal amounts of protein were analyzed by western blot using anti-PGIS, anti-COX-2, and anti-iNOS antibodies. Protein bands were visualized using horseradish peroxidase-conjugated secondary antibodies and electrochemiluminescence (ECL). (b) Inhibition of LPS-stimulated NO production, as nitrite release, in the medium by AdGFP and AdPGIS transduction in cultures. Control on -axis, no viral infection of naive cultures; open bar in chart indicates nonstimulated cultures. Closed bar indicates LPS-stimulated cultures. Data were expressed as means ± SEM from four independent experiments done in triplicate. indicates significant differences between nonstimulated and LPS-stimulated cultures within each Ad-transduced cells; AdPGIS + LPS compared with AdGFP + LPS.
