Lack of HXK2 Induces Localization of Active Ras in Mitochondria and Triggers Apoptosis in the Yeast Saccharomyces cerevisiae
Figure 2
hxk2Δ cells exhibit inhibition of cell growth and hypersensitivity to acetic acid. (a) W303-1A and hxk2Δ cells were harvested and resuspended (1-2 × 107 cells/mL) in SD medium adjusted either at pH 5.3 or at pH 3.0 (set with HCl) in the absence (NT) or in the presence of 40, 80, or 120 mM acetic acid. Cells were incubated for 200 min at 30°C with shaking (160 rpm). After treatment, cells were harvested and resuspended at the same concentration (108 cells/mL) in milliQ water. 5 microliter from a concentrated suspension and from 10-fold dilutions of each culture was spotted onto YEPD plates and incubated at 30°C for 3 days. (b) Cell survival of W303-1A and hxk2Δ strains. Cell viability of W303-1A and hxk2Δ untreated cells (▲) or treated with 80 mM (■) and 120 mM (⚫) acetic acid was analyzed at indicated times by measuring colony-forming units (cfu) after 2 days of growth at 30°C. Cell survival (100%) corresponds to the cfu at time zero. The means of 4 independent experiments with standard deviations are reported.
