Lack of HXK2 Induces Localization of Active Ras in Mitochondria and Triggers Apoptosis in the Yeast Saccharomyces cerevisiae

Figure 3

ROS accumulation in W303-1A, hxk2Δ, and hxk2Δ cells expressing the activated Ras2 ^Val19 allele after treatment with acetic acid. W303-1A (black bars), hxk2Δ (gray bars), and hxk2Δ cells expressing the activated Ras2^Val19 allele (white bars) exponentially growing cells were treated with different concentrations (40–80–120 mM) of acetic acid for 200 minutes at 30°C. ROS accumulation was assayed using the dye dihydrorhodamine 123 (DHR123) by flow cytometry. The means of 3 independent experiments with standard deviations are reported. Student’s 𝑡-test *𝑃<0.05 and **𝑃<0.01.