Lack of HXK2 Induces Localization of Active Ras in Mitochondria and Triggers Apoptosis in the Yeast Saccharomyces cerevisiae

Figure 4

The occurrence of acetic-acid-induced cell death in hxk2Δ cells is characterized by markers of both apoptosis and necrosis. Assessment of cell death by FITC-coupled annexin V and PI staining. W303-1A (black bars) and hxk2Δ (gray bars) exponentially growing cells were treated with different concentrations (40–80–120 mM) of acetic acid for 200 minutes at 30°C, before being processed for determination of phosphatidylserine externalization and membrane integrity by flow cytometry. 30000 events have been evaluated. The means of 3 independent experiments with standard deviations are reported. Student’s 𝑡-test *𝑃<0.05 and **𝑃<0.01.

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