Research Article

Lack of HXK2 Induces Localization of Active Ras in Mitochondria and Triggers Apoptosis in the Yeast Saccharomyces cerevisiae

Figure 6

Effect of YCA1 deletion on ROS accumulation after treatment with acetic acid. W303-1A (black bars), yca1Δ (black hatched bars), hxk2Δ (gray bars), and hxk2Δ-yca1Δ (white bars) exponentially growing cells were treated with different concentrations (40–80–120 mM) of acetic acid for 200 minutes at 30°C. ROS accumulation was assayed by flow cytometry using the dye dihydrorhodamine 123 (DHR123). Student’s -test * .
678473.fig.006