Potentiation of Antibiofilm Activity of Amphotericin B by Superoxide Dismutase Inhibition
Figure 5
Effect of DDC and ATM on ROS levels in C. albicans biofilms. (a) C. albicans CA-IF100 biofilms were treated with or without (/) 1 μM AmB in presence or absence (−) of 10 mM DDC or 10 mM ATM. Biofilms were washed with PBS and a sample was taken for CFU determination. Afterwards, 10 μM DCFDA was added. Fluorescence was normalized to the number of CFUs after treatment. (b) and (c). Effect of DDC on peroxide levels on C. albicans biofilm cells. C. albicans biofilms were treated with or without (/) 1 μM AmB in presence or absence (−) of 10 mM DDC. Biofilms were washed with PBS and a sample was taken for CFU determination. Afterwards, 20 μM DHE was added. Fluorescence was normalized to the number of CFUs after treatment. (b) CA-IF100, (c) F17 (white bars), and G6 (black bars). Data represent the mean and SEM for one representative experiment out of two, each consisting of triplicate measurements. *, ***.
