The P66Shc/Mitochondrial Permeability Transition Pore Pathway Determines Neurodegeneration

Figure 1

(a) P66Shc expression in different cell lines and spinal cord tissue. Western blot analysis of p66Shc was performed on whole-cell extracts from primary mouse embryonic fibroblasts (MEF), NG108, Kelly (human neuroblastoma), N2A (mouse), PC12(rat), SH-SY5Y (human neuroblastoma), NIH cells lines, and protein extract from mouse spinal cord. (b) Expression of p66Shc (upper panel) after UV treatment and (lower panel) levels of PhopsorylatedSer36-p66Shc in Kelly cells. (c) Expression of p66Shc upon specific p66Shc RNAi Interference on Kelly and response 20 μM H₂O₂ and 0.1 mM Staurosporine.

(a)

(b)

(c)