The P66Shc/Mitochondrial Permeability Transition Pore Pathway Determines Neurodegeneration
Figure 2
(a) ELISA assay for anti-MOG35–55 antibodies. (b) Proliferation assay. Cells from spleens, stimulated with MOG, were cocultured for 48 h and were radiolabelled with 9,25 kBq well-1 of methyl-3H thymidine in the last 16 h of coculture. The cells are harvested and counted in a liquid scintillation counter. (c) Cytokines production in medium of splenocytes from WT and p66Shc−/− mice. Results are representative of one of two experiments, each with five mice per group.
