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Oxidative Medicine and Cellular Longevity
Volume 2013 (2013), Article ID 780719, 7 pages
Research Article

Exhaustive Training Increases Uncoupling Protein 2 Expression and Decreases Bcl-2/Bax Ratio in Rat Skeletal Muscle

W. Y. Liu,1 W. He,2 and H. Li3

1Department of Sports Medicine, Shanghai University of Sport, Shanghai 200438, China
2Department of Rehabilitation, Shanghai Jing’an Geriatric Hospital, Shanghai 200040, China
3Department of Physical Education, Neijiang Normal University, Sichuan 641100, China

Received 17 October 2012; Revised 27 November 2012; Accepted 19 December 2012

Academic Editor: Emilio Luiz Streck

Copyright © 2013 W. Y. Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This work investigates the effects of oxidative stress due to exhaustive training on uncoupling protein 2 (UCP2) and Bcl-2/Bax in rat skeletal muscles. A total of 18 Sprague-Dawley female rats were randomly divided into three groups: the control group (CON), the trained control group (TC), and the exhaustive trained group (ET). Malondialdehyde (MDA), superoxide dismutase (SOD), xanthine oxidase (XOD), ATPase, UCP2, and Bcl-2/Bax ratio in red gastrocnemius muscles were measured. Exhaustive training induced ROS increase in red gastrocnemius muscles, which led to a decrease in the cell antiapoptotic ability (Bcl-2/Bax ratio). An increase in UCP2 expression can reduce ROS production and affect mitochondrial energy production. Thus, oxidative stress plays a significant role in overtraining.