Major Components of Energy Drinks (Caffeine, Taurine, and Guarana) Exert Cytotoxic Effects on Human Neuronal SH-SY5Y Cells by Decreasing Reactive Oxygen Species Production

Figure 9

Antioxidant enzyme activity and oxidative damage parameters on human neuronal SH-SY5Y cells exposed to major components of EDs. Enzymatic activity (SOD, CAT, GPx, and GST), SH, and TBARS measurements on guarana-treated cells (2 hours) at concentrations of 3.125, 6.25, 12.5, 25, and 50 mg/mL (a), caffeine-treated cells (2 hours) at concentrations of 0.125, 0.25, 0.5, 1, and 2 mg/mL (b), and taurine-treated cells (2 hours) at concentrations of  1, 2, 4, 8, and 16 mg/mL (c). Values are expressed as mean ± SEM of three independent experiments (𝑛=3). Statistical difference compared to control was determined by one-way ANOVA followed by Dunnett’s multiple comparison test (^∗𝑃<0.05; ^∗∗𝑃<0.01; ^∗∗∗𝑃<0.001).

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