The NRF2 Activation and Antioxidative Response Are Not Impaired Overall during Hyperoxia-Induced Lung Epithelial Cell Death
Figure 3
The effects of chronic hyperoxia on NRF2 target gene expression in AECs. Cells were exposed to either room air or hyperoxia for 36 or 48 h and then harvested for total RNA and protein. (a) HMOX1, GCLC, and NQO mRNA expression. The values represent the mean ± SD of four independent experiments (). , RA versus hyperoxia. (b) Western blot analysis of whole-cell lysates using anti-HMOX1, anti-NQO1, or anti-GCLC antibodies. Membranes were stripped and reprobed with -actin. Graph represents the relative band intensities of HMOX1, NQO, or GCLC, as quantified in Figure 2. Values represent the mean ± SD of four independent experiments (). , RA versus hyperoxia.
