Magnesium Can Protect against Vanadium-Induced Lipid Peroxidation in the Hepatic Tissue

Table 2

Main and interactive effects of V_end, Mg_end, Fe_exg, V_exg and Mg_exg on the MDA level measured in liver supernatants obtained from the SMV-MS coapplied rats incubated in in vitro conditions with Fe_exg30𝜇M, V_{exg100,200,400𝜇M} or Mg_{exg100,200,400𝜇M}.

Three-way ANOVA analysis^a		Character of interaction revealed or character of a trend toward interaction
LPO modified by Feexg30𝜇M
Main effect of Vend	𝐹=18.412, P = 0.000	—
Main effect of Mgend	𝐹=11.626, P = 0.001	—
Interactive effect of Vend  ×  Mgend	𝐹=8.097, P = 0.006	Antagonistic^b
Main effect of Feexg30𝜇M	𝐹=17.994, P = 0.000	—
Interactive effect of Feexg30𝜇M ×  Vend	F = 12.317, P = 0.001	Synergistic^c
Interactive effect of Feexg30𝜇M  ×  Mgend	F = 6.995, P = 0.010	Antagonistic^d
Interactive effect of Feexg30𝜇M  ×  Vend  ×  Mgend	F = 5.526, P = 0.021
LPO modified by Vexg100𝜇M
Main effect of Vend	F = 75.402, P = 0.000	—
Main effect of Mgend	F = 29.066, P = 0.000	—
Interactive effect of Vend  ×  Mgend	F = 6.950, P = 0.051	Antagonistic^b
Main effect of Vexg100𝜇M	F = 206.252, P = 0.000	—
Interactive effect of Vexg100𝜇M  ×  Vend	F = 46.205, P = 0.000	Synergistic^e
Interactive effect of Vexg100𝜇M  ×  Mgend	F = 12.570, P = 0.001	Antagonistic^f
Interactive effect of Vexg100𝜇M  ×  Vend  ×  Mgend	NS	—
LPO modified by Vexg200𝜇M
Main effect of Vend	F = 51.672, P = 0.000	—
Main effect of Mgend	F = 16.998, P = 0.000	—
Interactive effect of Vend  ×  Mgend	F = 2.999, P = 0.088	Antagonistic^b
Main effect of Vexg200𝜇M	F = 372.550, P = 0.000	—
Interactive effect of Vexg200𝜇M  ×  Vend	F = 35.037, P = 0.000	Synergistic^e
Interactive effect of Vexg200𝜇M  ×  Mgend	F = 8.299, P = 0.005	Antagonistic^f
Interactive effect of Vexg200𝜇M  ×  Vend  ×  Mgend	NS	—
LPO modified by Vexg400𝜇M
Main effect of Vend	F = 61.594, P = 0.000	—
Main effect of Mgend	F = 14.220, P = 0.000	—
Interactive effect of Vend  ×  Mgend	F = 5.271, P = 0.025	Antagonistic^b
Main effect of Vexg400𝜇M	F = 1026.907, P = 0.000	—
Interactive effect of Vexg400𝜇M  ×  Vend	F = 42.650, P = 0.000	Synergistic^e
Interactive effect of Vexg400𝜇M  ×  Mgend	F = 6.158, P = 0.015	Antagonistic^f
Interactive effect of Vexg400𝜇M  ×  Vend  ×  Mgend	NS	—
LPO modified by Mgexg100𝜇M
Main effect of Vend	F = 38.869, P = 0.000	—
Main effect of Mgend	F = 49.991, P = 0.000	—
Interactive effect of Vend  ×  Mgend	F = 12.331, P = 0.001	Antagonistic^b
Main effect of Mgexg100𝜇M	NS	—
Interactive effect of Mgexg100𝜇M  ×  Vend	NS	—
Interactive effect of Mgexg100𝜇M  ×  Mgend	NS	—
Interactive effect of Mgexg100𝜇M  ×  Vend  ×  Mgend	NS	—
LPO modified by Mgexg200𝜇M		—
Main effect of Vend	F = 39.615, P = 0.000	—
Main effect of Mgend	F = 48.829, P = 0.000	—
Interactive effect of Vend  ×  Mgend	F = 16.616, P = 0.000	Antagonistic^b
Main effect of Mgexg200𝜇M	F = 4.838, P = 0.031	—
Interactive effect of Mgexg200𝜇M  ×  Vend	NS	—
Interactive effect of Mgexg200𝜇M  ×  Mgend	NS	—
Interactive effect of Mgexg200𝜇M  ×  Vend  ×  Mgend	NS	—
LPO modified by Mgexg400𝜇M
Main effect of Vend	𝐹=41.824, 𝑃=0.000	—
Main effect of Mgend	𝐹=53.944, 𝑃=0.000	—
Interactive effect of Vend  ×  Mgend	𝐹=10.024, 𝑃=0.002	Antagonistic^b
Main effect of Mgexg400𝜇M	𝐹=10.024, 𝑃=0.002	—
Interactive effect of Mgexg400𝜇M  ×  Vend	NS	—
Interactive effect of Mgexg400𝜇M  ×  Mgend	NS	—
Interactive effect of Mgexg400𝜇M  ×  Vend  ×  Mgend	NS	—

V_end and Mg_end: endogenous V (NaVO₃, SMV) and Mg (MgSO₄, MS) which were received in combination for 18 weeks; Fe_exg, V_exg, Mg_exg: exogenous Fe (FeSO₄), V (NaVO₃) and Mg (MgSO₄) added to liver supernatants obtained from the SMV-MS-coadministered rats.
^aData are presented as 𝐹 values and the levels of significance (𝑃). NS: no significant effect.
^bThe effect of V_end and Mg_end in combination in the presence of Fe_exg30𝜇M or V_{exg100,200,400𝜇M} or Mg_{exg100,200,400𝜇M} < sum of the effects of V_end and Mg_end alone in the presence of Fe_{exg30𝜇𝑀} or V_{exg100,200,400𝜇M} or Mg_{exg100,200,400𝜇M} (antagonistic interaction).
^cThe effect of V_end and Fe_exg30𝜇M  in combination in the presence of Mg_end > sum of the effects of V_end and Fe_exg30𝜇M  alone in the presence of Mg_end (synergistic interaction).
^dThe effect of Mg_end and Fe_exg30𝜇M  in combination in the presence of V_end < sum of the effects of Mg_end and Fe_exg30𝜇M  alone in the presence of V_end (antagonistic interaction).
^eThe effect of V_end and V_{exg100,200,400𝜇M}  in combination in the presence of Mg_end > sum of the effects of V_end and V_{exg100,200,400𝜇M}  alone in the presence of Mg_end (synergistic interaction).
^fThe effect of Mg_end and V_{exg100,200,400𝜇M}  in combination in the presence of V_end < sum of the effects of Mg_end and V_{exg100,200,400𝜇M}  alone in the presence of V_end (antagonistic interaction).