Ethanol and Acetate Acting as Carbon/Energy Sources Negatively Affect Yeast Chronological Aging
Figure 4
Ethanol affects the glyoxylate-requiring gluconeogenesis and the respiration of cells in extreme CR. At the indicated time-points, Icl1 (a) and Pck1 (b) enzymatic activities were measured in wt cells during chronological aging in their expired medium and after the switch to water or water/ethanol as in Figure 3. Day 0, diauxic shift. (c) Cellular respiration of the same cells in the indicated experimental conditions. Error bars are the standard deviation of three replicates. (d) Chronologically aging wt cultures at the indicated time-points were serially diluted, plated onto YEPD and YEPG plates, and the index of respiratory competence (IRC) was determined. Standard deviations of three independent experiments are indicated.
