Research Article

Nitro-Oxidative Stress after Neuronal Ischemia Induces Protein Nitrotyrosination and Cell Death

Figure 3

Differential expression of mRNA NOS types occurs in different cell types. Cells were challenged with ischemia, and iNOS, eNOS, and nNOS mRNA expression were studied immediately at time 0, 12 and 24 h after the ischemic challenge. The expression of mRNA was assessed by semiquantitative RT-PCR, and bands were quantified by HPRT densitometric analysis in all cell types. Data are mean ± SEM values of 3 experiments in all cell types. ; versus controls at 0 h.
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