Nitro-Oxidative Stress after Neuronal Ischemia Induces Protein Nitrotyrosination and Cell Death

Figure 4

ONOO⁻ induces cell death. (a) Human neuroblastoma cells were treated with increasing concentrations of a NO donor (SNP) and H₂O₂ (free radical source). Cells were incubated for 6 h, and cell viability was assayed by MTT reduction. Data are expressed as percentage of control cells. Data are mean ± SEM values of 7 experiments. ^∗𝑃<0.05; ^∗∗𝑃<0.01 versus controls without SNP. (b) Cells were challenged with increasing concentrations of the ONOO⁻ donor SIN-1, and cell viability was assayed by MTT reduction.

(a)

(b)