Thiol Redox Sensitivity of Two Key Enzymes of Heme Biosynthesis and Pentose Phosphate Pathways: Uroporphyrinogen Decarboxylase and Transketolase

<table>Scheme for recombinant protein redox analysis. (1) Proteins are initially reduced with TCEP and an aliquot is removed and blocked with NEM; (2) the remaining protein is glutathionylated with a high concentration of GSSG, and an aliquot is removed and any remaining free thiols are blocked with NEM; (3) the protein aliquots removed are desalted and incubated with the Grx2p system and newly formed free thiols are blocked with NEM. Aliquots prepared are used for analysis by MS/MS and free thiol determinations using DTNB.</table>

Oxidative Medicine and Cellular Longevity

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Figure 2: Thiol Redox Sensitivity of Two Key Enzymes of Heme Biosynthesis and Pentose Phosphate Pathways: Uroporphyrinogen Decarboxylase and Transketolase 

Figure 2 | Thiol Redox Sensitivity of Two Key Enzymes of Heme Biosynthesis and Pentose Phosphate Pathways: Uroporphyrinogen Decarboxylase and Transketolase 