Thiol Redox Sensitivity of Two Key Enzymes of Heme Biosynthesis and Pentose Phosphate Pathways: Uroporphyrinogen Decarboxylase and Transketolase
Figure 7
Redox interconversion and activity of Tkl1p. (a) Representative extracted ion chromatogram and fragmentation (inset) of tryptic peptide from Tkl1p containing Cys622 (reduced) after treatment with TCEP (red) or GSSG (blue), followed by NEM. (b) Progenesis quantification of reduced peptide after treatments in the presence of Grx2p system (values in arbitrary units × 106). (c) Transketolase activity after redox treatments, ≈80% inhibition of enzyme activity after pretreatment with NEM, significance . *The final concentration of NEM in the assay was 0.01 mM; this concentration had little effect on activity. Units are μmol min−1/μg protein.
