Short-Term Diet and Moderate Exercise in Young Overweight Men Modulate Cardiocyte and Hepatocarcinoma Survival by Oxidative Stress
Figure 1
Effect of overweight young man sera on cell number, oxidative stress, and neutral lipid accumulation in H9C2 cardiocyte cell line. (a) H9C2 cell number exposed for 72 h to pre- and postintervention sera. (b) NO levels assessed by Griess method in the medium of H9C2 cells exposed for 72 h to pre- and postintervention sera. (c) Mitochondrial superoxide anion production in H9C2 cells exposed for 72 h to pre- and postintervention sera was analysed by HE (20 ng/mL) staining. Dye accumulation was analysed with FACScan flow cytometer (FACScan, Becton Dickinson) by the CellQuest software. For each sample, 2 × 104 events were acquired. (d) Neutral lipid accumulation evaluated by ORO-based colorimetric assay in H9C2 cells exposed for 72 h to pre- and postintervention sera. Control (Ctr) was assumed to be cells cultured with 10% fetal bovine serum. All analyses were carried out in triplicate from at least three separate experiments. Values, means. Bars, SDs. denotes , denotes , and denotes .
