Short-Term Diet and Moderate Exercise in Young Overweight Men Modulate Cardiocyte and Hepatocarcinoma Survival by Oxidative Stress

Figure 2

Effects of overweight young man sera on total ERK1/2, phosphorylated ERK1/2 (p-ERK1/2), Hsp27, Hsp90, and tubulin expression evaluated by western blotting analysis in H9C2 cells. H9C2 cells were treated for 72 h with either 10% fetal bovine serum (Ctr) or pre- (pre) or postintervention (post) sera. The bands associated with ERK1/2, phosphorylated ERK1/2 (p-ERK), Hsp27, Hsp90, and house-keeping tubulin expression were opportunely visualized as explained in Section 2.4.2. The expression of the house-keeping protein tubulin was used as loading control. The experiments were repeated several times and gave always similar results.