Short-Term Diet and Moderate Exercise in Young Overweight Men Modulate Cardiocyte and Hepatocarcinoma Survival by Oxidative Stress
Figure 3
Effect of overweight young man sera on proliferation, oxidative stress, and neutral lipid accumulation in HepG2 cell line. (a) Cell number of HepG-2 exposed for 72 h to pre- and postintervention sera; (b) NO level assessed by Griess method in the medium of HepG-2 cells exposed for 72 h to pre- and postintervention sera. (c) Lipid peroxidation levels assessed using the TBARS method in HepG-2 cells exposed for 72 h to pre- and postintervention sera. (d) Neutral lipid accumulation evaluated by ORO-based colorimetric assay in HepG-2 cells exposed for 72 h to pre- and postintervention sera. Control (Ctr) was assumed to be cells cultured with 10% fetal bovine serum. All analyses were carried out in triplicate from at least three separate experiments. Values, means. Bars, SDs. denotes , denotes , and denotes .
