Cucurbitacin E Has Neuroprotective Properties and Autophagic Modulating Activities on Dopaminergic Neurons

Figure 11

Immunofluorescence detection of several cytoskeletal proteins in CuE- or vehicle-treated neuronal cells (Ctrl). Cells were stained for β-actin (red), β3-tubulin (green), or neurofilament-M (NF-M) (purple). Hoechst 33342 (blue) was used to counterstain all nuclei. CuE treatment for 24 hours does not visibly alter the neuronal cytoskeleton. Microphotographs are representative of 3 different experiments. Scale bar = 10 μm.