Astragalus Polysaccharide Suppresses Doxorubicin-Induced Cardiotoxicity by Regulating the PI3k/Akt and p38MAPK Pathways

Figure 2

Doxorubicin induces cell injury by promoting apoptosis in cardiomyocytes. (a) The cell viability NRVMs cultured with different concentrations of doxorubicin (0, 0.1, 0.5, 1, 3, 5, 10, 20, and 30 μM) for 24 h as demonstrated by MTT assay (). (b) Representative TUNEL staining of NRVMs cultured with different concentrations of doxorubicin (0, 0.1, 0.5, 1, 3, and 5 μM) for 24 h. (c) Average TUNEL staining data (). (d) Dose response of activated (cleaved) caspase 3 and phosphorylated p38MAPK as assayed by Western blotting for NRVMs treated with 0.1–5.0 μM doxorubicin for 24 h () (, , and versus the control group).

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