Astragalus Polysaccharide Suppresses Doxorubicin-Induced Cardiotoxicity by Regulating the PI3k/Akt and p38MAPK Pathways

Figure 3

APS reverses the doxorubicin-induced oxidative stress and apoptosis of cardiomyocytes. (a) DHE staining of control NRVMs, doxorubicin-treated (1 μM) NRVMs, and NRVMs pretreated with APS (50 μg/mL) followed by doxorubicin treatment (, ). (b) Cardiomyocyte apoptosis as detected by TUNEL in control and doxorubicin-treated (1 μM) NRVMs and NRVMs pretreated with APS (50 μg/mL) followed by doxorubicin treatment (). (c) Cardiomyocyte apoptosis as detected by DNA laddering for control and doxorubicin-treated (1 μM) NRVMs and NRVMs pretreated with APS (50 μg/mL) followed by doxorubicin treatment (). (d) APS suppressed doxorubicin-induced caspase 3 and caspase 9 activation in a concentration-dependent manner in NRVMs as assayed by Western blotting () ( versus control group, versus the doxorubicin-treated group, and versus the doxorubicin-treated group).

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