Expression of Senescence-Associated microRNAs and Target Genes in Cellular Aging and Modulation by Tocotrienol-Rich Fraction
Figure 3
Effect of TRF treatment on the expression level of miR-34a in nontransfected young HDFs, young HDFs transfected with miR-34a mimic, miRNA negative control, and senescent HDFs measured by real time qRT-PCR. Young HDFs were transfected with miR-34a mimic (10 nM) to overexpress miR-34a or miRNA negative control as control for 24 h, followed by TRF treatment for 24 h. compared to control young untransfected HDFs, compared to young HDFs transfected with miRNA negative control, and compared to control senescent HDFs. Data are presented as relative expression value (REV) normalized to RNU6B expression (mean ± SD, ).
