Expression of Senescence-Associated microRNAs and Target Genes in Cellular Aging and Modulation by Tocotrienol-Rich Fraction

Figure 4

Effect of TRF treatment on the expression level of CDK4 (a), CCND1 (b), SIRT1 (c), and TP53 (d) in nontransfected young HDFs, young HDFs transfected with miR-34a mimic, miRNA negative control, and senescent HDFs measured by real time qRT-PCR. ^adenotes𝑃<0.05 compared to control young untransfected HDFs, ^b𝑃<0.05 compared to control young HDFs transfected with miR-34a mimic, ^c𝑃<0.05 compared to control young HDFs transfected with miRNA negative control, and ^d𝑃<0.05 compared to control senescent HDFs. Data are presented as relative expression value (REV) normalized to GAPDH expression (mean ± SD, 𝑛=6).

(a)

(b)

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(d)