Expression of Senescence-Associated microRNAs and Target Genes in Cellular Aging and Modulation by Tocotrienol-Rich Fraction

Table 2

Primers sequences for quantitative gene expression analysis.

Accession number	Gene	Primer	Primer sequences; (5′→3′)	PCR product size (bp)
NM_002046	GAPDH	Forward	TCCCTGAGCTGAACGGGAAG	217
	GAPDH	Reverse	GGAGGAGTGGGTGTCGCTGT
NM_053056	CCND1	Forward	AGACCTTCGTTGCCCTCTGT	181
	CCND1	Reverse	CAGTCCGGGTCACACTTGAT
NM_000075	CDK4	Forward	TGGCCCTCAAGAGTGTGAGA	147
	CDK4	Reverse	ATGTGGCACAGACGTCCATC
NM_012238	SIRT1	Forward	GCAGATTAGTAGGCGGCTTG	152
	SIRT1	Reverse	TCTGGCATGTCCCACTATCA
NM_000546	TP53	Forward	GGAAGAGAATCTCCGCAAGAA	177
	TP53	Reverse	AGCTCTCGGAACATCTCGAAG