PRAK Interacts with DJ-1 and Prevents Oxidative Stress-Induced Cell Death

Figure 1

Interaction of DJ-1 and PRAK in yeast. (a) Myc-DBD-PRAK fusion protein detected by Western blot using an antibody against Myc. (b) The coding sequence of a positive clone was identical to the C-terminal (174~567 bp) of Homo sapiens DJ-1. The underdot-lined region represents the coding sequence (58–189 aa) of the positive clone that interacts with PRAK. (c) and (d) β-Galactosidase activity of yeast clones either expressing AD-DJ-1 and DBD-PRAK fusion proteins (c) or expressing AD-DJ-1 and DBD proteins (d) on SD/-Ade/-His/-Leu/-Trp/X-α-gal plates.

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